Not really, staining cells is done once the cells are preserved using a type of formaldehyde so the cells shouldn’t change at all, the fluorescent antibodies we use just help us see the part we’re interested in. The antibodies ‘stick’ to the target we’re interested in and will light up when we shine different lasers at it under a microscope. You can do some of this with live cells, in which case they might react differently, but all of mine have been done in fixed – or preserved – cells.
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